Analysis Note

Positive ControlEndothelial cells, Kupffer cells, or macrophages

Application

Immunoblotting (1:200-1:400)r>Immunoprecipitation (10 µl/mg protein lysate)

General description

Recognizes the ~82 kDa hepatocyte growth factor protein in macrophages, endothelial, and Kupffer cells.

Purified mouse monoclonal antibody. Recognizes the ~82 kDa HGF protein.

Anti-HGF (Scatter Factor) (Ab-1), mouse monoclonal, clone 4C12.1, recognizes the ~82 kDa hepatocyte growth factor in macrophages, endothelial & Kupffer cells. It is validated for use in WB & IP.

Immunogen

recombinant, human HGF protein

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Toi, M., et al. 1998. Clin. Cancer Res.4, 659.Bardelli, A. and Comoglio, P.M. 1997. Ciba Found. Symp.212, 133.Birchmeier, C., et al. 1997. Ciba Found. Symp.212, 16.Jiang, W.G. and Hiscox, S. 1997. Histol. Histopathol.12, 537.Matsumoto, K. and Nakamura, T. 1997. Ciba Found. Symp.212, 198.Rosen, E.M., et al. 1997. Ciba Found. Symp.212, 215.Vande Woude, G.F., et al. 1997. Ciba Found Symp.212, 119.

Antibody should be titrated for optimal results in individual systems.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 10 mM PBS, 0.2% BSA, pH 7.4.

Reconstitution

Following initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.

Warning

Toxicity: Standard Handling (A)